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| 3. |  | RAIMUNDO, J. F. S.; MORATÓ, R.; ARCARONS, N.; YESTE, M.; DODE, M. A.; LÓPEZ-BEJAR, M.; MOGAS, T. Assessment of the effect of adding L-carnitine and/or resveratrol to maturation medium before vitrification on in vitro-matured calf oocytes. Theriogenology, v. 89, 47-57, 2017.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
04/09/2019 |
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Data da última atualização: |
13/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
OKINO, C. H.; GIGLIOTI, R.; BASSETTO, C. C.; SILVA, P. C.; OLIVEIRA, H. N. de; OLIVEIRA, M. C. de S. |
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Afiliação: |
CINTIA HIROMI OKINO, CPPSE; Rodrigo Giglioti, Instituto de Zootecnia; César Cristiano Bassetto, UNESP; Pamella Cristini Silva, UNICEP; Henrique Nunes de Oliveira, Instituto de Zootecnia; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE. |
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Título: |
Elimination of erroneous results related to bovine mononuclear cell immunophenotyping by antibodies binding to Fc receptors. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Veterinary Immunology and Immunopathology, v. 213, jul. 2019. |
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DOI: |
https://doi.org/10.1016/j.vetimm.2019.109889 |
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Idioma: |
Inglês |
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Conteúdo: |
Blocking immunoglobulin G (IgG) binding receptors on leukocytes is an established and highly recommended preventive procedure for immunological assays. Failing to prevent such nonspecific binding can lead to erroneous results. Several studies testing different blocking reagents have been performed in murine or human cells, however, there are no specific studies on bovine cells. Our study aimed to investigate the efficiency of blocking reagents to inhibit the nonspecific binding of mouse monoclonal antibodies (mAbs) to bovine peripheral blood cells. We observed nonspecific interactions of IgG2a and IgG2b negative isotypes with bovine leukocytes, but not IgG1. We found that these nonspecific bindings could be eliminated by blocking with purified mouse IgG, whereas little or no blocking effect was observed when bovine serum or Mouse Seroblock FcR were applied. Moreover, in the absence of an efficient blocking reagent, the percentage of CD335 positive cells was significantly higher than in the group previously blocked with mouse IgG. Based on these results, and due to the lack of specific commercial blocking reagents for bovine cells, our recommendation is to use purified mouse IgG as a blocking reagent for immune assays targeting bovine leukocytes in order to enhance the accuracy of the results. |
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Palavras-Chave: |
Ensaios imunológicos; Fc receptor blocking; Mouse IgG; Nonspecific antibody binding; RPE-Cy5. |
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Thesaurus NAL: |
Flow cytometry. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
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001 2111876
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024 7 $ahttps://doi.org/10.1016/j.vetimm.2019.109889$2DOI
100 1 $aOKINO, C. H.
245 $aElimination of erroneous results related to bovine mononuclear cell immunophenotyping by antibodies binding to Fc receptors.$h[electronic resource]
260 $c2019
520 $aBlocking immunoglobulin G (IgG) binding receptors on leukocytes is an established and highly recommended preventive procedure for immunological assays. Failing to prevent such nonspecific binding can lead to erroneous results. Several studies testing different blocking reagents have been performed in murine or human cells, however, there are no specific studies on bovine cells. Our study aimed to investigate the efficiency of blocking reagents to inhibit the nonspecific binding of mouse monoclonal antibodies (mAbs) to bovine peripheral blood cells. We observed nonspecific interactions of IgG2a and IgG2b negative isotypes with bovine leukocytes, but not IgG1. We found that these nonspecific bindings could be eliminated by blocking with purified mouse IgG, whereas little or no blocking effect was observed when bovine serum or Mouse Seroblock FcR were applied. Moreover, in the absence of an efficient blocking reagent, the percentage of CD335 positive cells was significantly higher than in the group previously blocked with mouse IgG. Based on these results, and due to the lack of specific commercial blocking reagents for bovine cells, our recommendation is to use purified mouse IgG as a blocking reagent for immune assays targeting bovine leukocytes in order to enhance the accuracy of the results.
650 $aFlow cytometry
653 $aEnsaios imunológicos
653 $aFc receptor blocking
653 $aMouse IgG
653 $aNonspecific antibody binding
653 $aRPE-Cy5
700 1 $aGIGLIOTI, R.
700 1 $aBASSETTO, C. C.
700 1 $aSILVA, P. C.
700 1 $aOLIVEIRA, H. N. de
700 1 $aOLIVEIRA, M. C. de S.
773 $tVeterinary Immunology and Immunopathology$gv. 213, jul. 2019.
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Embrapa Pecuária Sudeste (CPPSE) |
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